Resveratrol attenuates the development of nitro-oxidative stress in the liver of rats exposed to round-the-clock lighting and a high-carbohydrate-lipid diet

Abstract

The aim of this study is to investigate the effects of resveratrol on nitro-oxidative stress in the liver of rats exposed to round-the-clock lighting (RCL) and kept on the high-carbohydrate-lipid diet (HCLD). The experiment was performed on 21 Wistar white male rats weighing 215-255 g. Control animals (Group 1) were fed standard chow and kept on a 12/12 hour light/dark cycle. Rats of the Group 2 were kept on HCLD (20% aqueous fructose solution for drinking and an appropriate ration) for 8 weeks and exposed to RCL, thus simulating MS development. The animals of the Group 3 received resveratrol in a daily dose of 5 mg/kg intragastrically starting on the 30th day of the experiment. We evaluated the production of the superoxide anion radical (•O-2), total NO-synthase (NOS) activity, the activity of its constitutive and inducible NOS isoforms (cNOS/iNOS) and the concentration of peroxynitrites of alkali and alkali-earth metals. As a result, the administration of resveratrol under experimental conditions reduces the following nitro-oxidative stress indicators as the •O-2 production by microsomal monooxygenases, cNOS, mitochondrial respiratory chain, and phagocytic NADPH oxidase, the NOS activity (total and its inducible isoenzyme), and peroxynitrites concentration under the growing activity of the cNOS and its coupling index. In conclusion, the administration of resveratrol under constant impact of adverse factors of the Western lifestyle (a diet rich in fats and carbohydrates, disruptions of circadian rhythm) is an effective means to restrict the production of reactive oxygen and nitrogen species in the liver tissues.